Cell Counting Kit -8 IV08

CCK is a one-bottle solution;no premixing of components is required.CCK ,being nonradioactive, allows sensitive,Cell viability and cytotoxicity assays are used for drug screening and cytotoxicity tests of chemicals.Since WST-8 formazan is water soluble, it does not form crystals. Therefore, solubilizing process such as MTT assay is not required. Additionally, The detection sensitivity of CCK-8 is higher than the other tetrazolium salts such as MTT, XTT, MTS or WST-1,colorimetric assays for the determination of the number of viable cells in cell proliferation and cytotoxicity assays.

Number of Cells Determination

1.Inoculate cell suspension (100ul/well）in a 96-well plate.Pre-incubate the plate in a humidified incubator(e.g.,at 37℃，5% CO2).

2.Add 10ul of the CCK solution to each well of the plate.

Be careful not to introduce bubbles to the wells ,since they interfere with the O.D. Reading.

4. Incubate the plate for 1 - 4 hours in the incubator.

5. Measure the absorbance at 450 nm using a microplate reader.

To measure the absorbance later, add 10μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change  should be observed for 24 hours .  

Cell Proliferation and Cytotoxicity Assay

1.Dispense 100 μl of cell suspension (5000 cells/well) in a 96-well plate. Pre-incubate the plate for 24 hours in a  humidified incubator (e.g., at 37°C,5% CO2).

2.Add 10 μl of various concentrations of substances to be tested to the plate.

3.Incubate the plate for an appropriate length of time (e.g., 6, 12, 24 or 48 hours) in the incubator.

4.Add 10 μl of CCK solution to each well of the plate.

Be careful not to introduce bubbles to the wells, since they interfere with the O.D. reading.

5.Incubate the plate for 1 - 4 hours in the incubator.

6. Measure the absorbance at 450 nm using a microplate reader.

To measure the absorbance later, add 10 μl of 1% w/v SDS or 0.1 M HCl to each well, cover the plate and store it with protection from light at room temperature. No absorbance change should be observed for 24 hours.