Sterility : Sterile filtered
State : Frozen
Storage : -20°C
Shelf Life : 60 months
Shipping conditions : -20°C or dry ice
Our system of vertical integration allows us to be certain of the origins and traceability of our serum.
Each manufactured batch is rigorously controlled, from the collection of serum and throughout all
stages of its treatment and production through to final packaging on our premises.
invigentech Pig Serum is derived from clotted whole blood collected from pig with cleaned and
disinfected equipment. The blood is centrifuged and the supernatant called “serum” is put in jugs
The serum is collected or imported and treated in agreement with the European regulations.
Final Filter Size : 0.2 µm
All sera are tested for the absence of aerobic and anaerobic bacteria, fungi, yeast and Mycoplasma.
The sterility test is based on the European Pharmacopoeia requirements.
The sera are tested for the absence of Mycoplasma by culture
All sera are tested to determine the levels of endotoxins. BioWest carries out a chromokinetic
quantitative test, according to the method D of the European Pharmacopoeia.
The endotoxin reagent is standardized against the US reference endotoxin.
The haemoglobin level is measured by spectrophotometer.
pH specification: 7,3 to 8,3.
Determined by a lowered freezing temperature. The osmometer is calibrated against standard solution.
Biological performance is assessed using cell culture medium supplemented with the serum being
During the test period, cultures are examined microscopically for any morphological abnormalities
that may indicate toxic components in the serum.
Cell Culture Tests:
Cell Growth, Plating Efficiency, Cloning Efficiency.
Cell Lines Tested:
The following cell lines are tested with the serum:
L929- Fibroblast/Mouse/ As Macrophage
Determined by Biuret Colorimetry.
Country of Origin:
The country in which the serum was taken from the donor/animal.
invigentech sera are sourced from the following countries: France, Germany, Italy, Brazil,
Store at -20°C
Recommended use: - Respect storage conditions of the serum
- Do not use the serum after its expiry date
- Store serum in an area protected from light
- Manipulate serum in aseptic conditions (e.g. : under laminar air flow)
- Wear clothes adapted to the manipulation of serum to avoid contamination (e.g. : gloves, mask,
hygiene cap, overall…)
- In order to preserve all serum qualities, it is recommended to thaw out the flask, to aliquote, then to
re-freeze the produced flasks rather than to thaw out and re-freeze the flask at each use.
- It is recommended to use the serum immediately after its thaw out. However, if it is not useful, it is
possible to store thaw out serum, at +2°C / +8°C, until 26 weeks without significant decrease of its
performances in cell culture.
The product is intended to be used in vitro, in laboratory only. Do not use it in therapy, human or