SE series serum-free cell cryopreservation solution L1100

SE series serum-free cell cryopreservation solution

Overview

The usage method of SE series serum-free cell cryopreservation solution is very simple. Simply collect the cells to be frozen, add an appropriate amount of serum-free cell cryopreservation solution, mix well, and then directly store them in a -80 ℃ ultra-low temperature refrigerator without the need for programmed cooling or liquid nitrogen storage, nor do you need to prepare any solution immediately. Serum-free cell cryopreservation solution is suitable for the cryopreservation of most mammalian and human cells, including tumor cells, conventional cells, primary cells, stem cells, etc.

Packing specification

Product Numbers: L1100

Specification:  100ml

Storage conditions

Store at 4ºC, valid for one year; Store at -20ºC, valid for two years;

Product advantages

lNo program cooling

lReady-to-use cell cryopreservation solution

lNo animal-derived ingredients, no serum ingredients, and clear chemical ingredients

lFast freezing, can be placed directly in the refrigerator at -80 ℃

lHigh cell recovery rate

Some cells with cell recovery efficiency up to 90%

NCI-H460、CHO、COS7，RAW264.7、NIH-3T3、MCF-7、HEK-293、HepG2、Hela、C2C12、A549，DU145、MDCK、THP-1、MSC Wait。

Freezing operation steps

1. Collect suspended cells or adherent cells and centrifuge to remove the supernatant;

2. Add an appropriate amount of cell cryopreservation solution, resuspend the cells, and adjust the cell density to 5x10⁵-1x10⁷ / ml;

3. Dispense the cell suspension added with cryopreservation solution into cryopreservation tubes;

4. Directly transfer the cryopreservation tube to a refrigerator at -80ºC for frozen storage;

Precautions

1. Please choose to freeze the logarithmic growth phase cells;

2. After adding the cryopreservation solution to the cells, please put them into the -80ºC refrigerator for storage as soon as possible;

3. The frozen cells of this product can be stored in the refrigerator at -80ºC for more than 5 years;

4. If you need to freeze the cells for a long time, please transfer to a liquid nitrogen tank for storage;

The cryopreservation fluid contains DMSO. For your safety and health, please wear lab coat and disposable gloves

Frozen cell recovery steps

1. Thaw the cells in a 37 ° C water bath equipment to confirm that the cell fluid is completely thawed.

2. After the cell mixture in the cryotube is completely thawed, immediately add 1ml of cell culture medium to the cryotube and mix with the cells.

Transfer the mixed solution to a centrifuge tube containing about 5ml of the cell culture medium, 1000rmp, centrifuge for 5 minutes to collect the frozen

Save the cell pellet and remove the supernatant.

3. Add an appropriate amount of fresh cell culture medium, slowly add to the cell pellet using a pipette, mix gently, and mix the cells

The mixed solution is transferred to the culture container prepared in advance.

It can only be used for scientific research. It is forbidden to use it for human, animal or other purposes.